KAP1/TIF1β Mouse mAb_单克隆抗体-上海泊湾生物技术有限公司

KAP1/TIF1β Mouse mAb

Source: Mouse mAb Catalog No. : AB3132

KAP1/TIF1β Mouse mAb (AB3132)
UniProt:Q13263
Application:WB,IHC,ICC/IF,IP
Reactivity:Human
Source:Mouse mAbhttp://www.abways.cn/showproduct.asp?cid=AB3132

 Datasheet

数据表

UniProt

Q13263
Mol Weight

110KDa
Alternative Names

E3 SUMO-protein ligase TRIM28; KRAB-associated protein 1; KAP-1; KRAB-interacting protein 1; KRIP-1; Nuclear corepressor KAP-1; KAP1; TF1B; RNF96; TIF1B; TRIM28;
Immunogen

Purified recombinant human KAP1 / TIF1 beta protein fragments expressed in E.coli.
Storage

Mouse IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at +4°C short term. Store at -20°C long term. Avoid freeze / thaw cycle.
Purification

Affinity-chromatography
Isotype

Mouse IgG1
Clonality

Monoclonal
Description

Mediates gene silencing by recruiting CHD3, a subunit of the nucleosome remodeling and deacetylation (NuRD) complex, and SETDB1 (which specifically methylates histone H3 at 'Lys-9' (H3K9me)) to the promoter regions of KRAB target genes. Enhances transcriptional repression by coordinating the increase in H3K9me, the decrease in histone H3 'Lys-9 and 'Lys-14' acetylation (H3K9ac and H3K14ac, respectively) and the disposition of HP1 proteins to silence gene expression.

Applications

Reactivity

Human
Application

WB,IHC,ICC/IF,IP
Recommended dilution

WB 1:1000 IHC 1:500 ICC/IF 1:100 IP

Western blot detection of KAP1 / TIF1 beta in 293T and HepG2 cell lysates using KAP1 / TIF1 beta mouse mAb (1:1000 diluted).Observed band size: 110KDa.
IHC of paraffin-embedded human Spleen using anti-KAP1 / TIF1 beta diluted 1/500-1/1000.
Immunofluorescent analysis of HeLa cells fixed with 4% Paraformaldehyde and using anti-KAP1 / TIF1 beta mouse mAb (dilution 1:100).
Immunoprecipitation analysis of Hela cell lysates using KAP1 / TIF1 beta mouse mAb.