SMAD5(C-term) Mouse mAb_单克隆抗体-上海泊湾生物技术有限公司

SMAD5(C-term) Mouse mAb

Source: Mouse mAb Catalog No. : AB3154

SMAD5(C-term) Mouse mAb (AB3154)
UniProt:Q99717
Application:WB,ICC/IF,FC
Reactivity:Human
Source:Mouse mAbhttp://www.abways.cn/showproduct.asp?cid=AB3154

 Datasheet

数据表

UniProt

Q99717
Mol Weight

60KDa
Alternative Names

Dwfc; hSmad5; JV5-1 ; MADH5; SMAD5; MusMLP ;SMAD family member 5;
Immunogen

Purified recombinant human SMAD5 (C-terminus) protein fragments expressed in E.coli.
Storage

Mouse IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at +4°C short term. Store at -20°C long term. Avoid freeze / thaw cycle.
Purification

Affinity-chromatography
Isotype

Mouse IgG1
Clonality

Monoclonal
Description

Transcriptional modulator activated by BMP (bone morphogenetic proteins) type 1 receptor kinase. SMAD5 is a receptor-regulated SMAD (R-SMAD).

Applications

Reactivity

Human
Application

WB,ICC/IF,FC
Recommended dilution

WB 1:1000 ICC/IF 1:75 FC 1:100

Western blot detection of SMAD5 (C-terminus) in Hela,Jurkat and K562 cell lysates using SMAD5 (C-terminus) mouse mAb (1:1000 diluted).Predicted band size:52KDa.Observed band size:60KDa.
Immunofluorescent analysis of HeLa cells using anti-SMAD5 (C-terminus) mouse mAb diluted 1:75.
Flow Cytometry analysis of Jurkat cells stained with SMAD5 (red, 1/100 dilution), followed by FITC-conjugated goat anti-mouse IgG. Black line histogram represents the isotype control, normal mouse IgG.