GSK3 β Mouse mAb_单克隆抗体-上海泊湾生物技术有限公司

GSK3 β Mouse mAb

Source: Mouse mAb Catalog No. : AB3168

GSK3 β Mouse mAb (AB3168)
UniProt:P49841
Application:WB,ICC/IF,IP
Reactivity:Human,Mouse
Source:Mouse mAbhttp://www.abways.cn/showproduct.asp?cid=AB3168

 Datasheet

数据表

UniProt

P49841
Mol Weight

46KDa
Alternative Names

Glycogen Synthase Kinase 3 Beta; Glycogen synthase kinase-3 beta; GSK 3 beta; GSK-3 beta; GSK3B; GSK3beta isoform;
Immunogen

Purified recombinant GSK-3β protein fragments expressed in E.coli.
Storage

Mouse IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at +4°C short term. Store at -20°C long term. Avoid freeze / thaw cycle.
Purification

Affinity-chromatography
Isotype

Mouse IgG2a
Clonality

Monoclonal
Description

Participates in the Wnt signaling pathway. Implicated in the hormonal control of several regulatory proteins including glycogen synthase, MYB and the transcription factor JUN. Phosphorylates JUN at sites proximal to its DNA-binding domain, thereby reducing its affinity for DNA. Phosphorylates MUC1 in breast cancer cells, and decreases the interaction of MUC1 with CTNNB1/beta-catenin. Phosphorylates CTNNB1/beta-catenin.

Applications

Reactivity

Human,Mouse
Application

WB,ICC/IF,IP
Recommended dilution

WB 1:1000 ICC/IF 1:200 IP

Western blot analysis of extracts from A549, Hela, 3T3 and Rat Brain cell lysates using GSK-3β mouse mAb(1:1000 diluted).Predicted band size:46KDa.Observed band size:46KDa.
Immunofluorescent analysis of HeLa cells fixed with -20℃ Methanol and using GSK-3β mouse mAb (dilution 1:200).
Immunoprecipitation analysis of Hela cell lysates using GSK-3β.GSK-3β Rabbit mAb was used for the western blot analysis (1:1000 diluted).