MECT1/Torc1 Mouse mAb_单克隆抗体-上海泊湾生物技术有限公司

MECT1/Torc1 Mouse mAb

Source: Mouse mAb Catalog No. : AB3184

MECT1/Torc1 Mouse mAb (AB3184)
UniProt:Q6UUV9
Application:WB,ICC/IF,IP,FC
Reactivity:Human
Source:Mouse mAbhttp://www.abways.cn/showproduct.asp?cid=AB3184

 Datasheet

数据表

UniProt

Q6UUV9
Mol Weight

78KDa
Alternative Names

CREB regulated transcription coactivator 1; CRTC1; MECT 1;Mucoepidermoid carcinoma translocated 1; TORC1;Transducer of CREB protein 1;
Immunogen

Purified recombinant human MECT1 / Torc1 protein fragments expressed in E.coli.
Storage

Mouse IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at +4°C short term. Store at -20°C long term. Avoid freeze / thaw cycle.
Purification

Affinity-chromatography
Isotype

Mouse IgG2b
Clonality

Monoclonal
Description

Transcriptional coactivator for CREB1 which activates transcription through both consensus and variant cAMP response element (CRE) sites. Acts as a coactivator, in the SIK/TORC signaling pathway, being active when dephosphorylated and acts independently of CREB1 'Ser-133' phosphorylation.

Applications

Reactivity

Human
Application

WB,ICC/IF,IP,FC
Recommended dilution

WB 1:1000 ICC/IF 1:300 IP FC 1:100

Western blot detection of MECT1 / Torc1 in Hela and Jurkat lysates using MECT1 / Torc1 mouse mAb (1:1000 diluted).Predicted band size: 78KDa.Observed band size: 78KDa.
Immunofluorescent analysis stain of Hela using MECT1 / Torc1 mouse mAb (1:300).
Immunoprecipitation analysis of Hela cell lysate using MECT1 / Torc1 mouse mAb.
Flow Cytometry analysis of K562 cells stained with TORC1(N-terminus) (red, 1/100 dilution), followed by FITC-conjugated goat anti-mouse IgG. Blue line histogram represents the isotype control, normal mouse IgG.