eEF2 Mouse mAb
Source: Mouse mAb Catalog No. : AB3190
eEF2 Mouse mAb (AB3190)
UniProt:P13639
Application:WB,ICC/IF
Reactivity:Human
Source:Mouse mAbhttp://www.abways.cn/showproduct.asp?cid=AB3190
数据表
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UniProt
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Mol Weight95KDa
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Alternative NamesEEF 2; Eef2; EF-2; EF2; Elongation factor 2; Polypeptidyl tRNA translocase; SCA26;
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ImmunogenPurified recombinant human eEF2 protein fragments expressed in E.coli.
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StorageMouse IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at +4°C short term. Store at -20°C long term. Avoid freeze / thaw cycle.
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PurificationAffinity-chromatography
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IsotypeMouse IgG2b
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ClonalityMonoclonal
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DescriptionCatalyzes the GTP-dependent ribosomal translocation step during translation elongation. During this step, the ribosome changes from the pre-translocational (PRE) to the post-translocational (POST) state as the newly formed A-site-bound peptidyl-tRNA and P-site-bound deacylated tRNA move to the P and E sites, respectively.
Applications
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ReactivityHuman
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ApplicationWB,ICC/IF
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Recommended dilutionWB 1:5000 ICC/IF 1:200
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Western blot detection of eEF2 in HL-60,Jurkat,SHSY-5Y,U20S and Hela cell lysates using eEF2 mouse mAb (1:5000 diluted).Predicted band size:95KDa.Observed band size: 95KDa.Exposure time:15s. -
Immunofluorescent analysis of HeLa cells fixed with -20℃ Methanol and using anti-eEF2 mouse mAb (dilution 1:200).
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