SAFB1 Mouse mAb
Source: Mouse mAb Catalog No. : AB3195
SAFB1 Mouse mAb (AB3195)
UniProt:Q15424
Application:WB,ICC/IF
Reactivity:Human
Source:Mouse mAbhttp://www.abways.cn/showproduct.asp?cid=AB3195
数据表
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UniProt
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Mol Weight130KDa
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Alternative NamesHAP; HET;HSP27 ERE TATA binding protein; HSP27 ERE-TATA-binding protein; SAF-B1; SAFB1; scaffold attachment factor B1;
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ImmunogenPurified recombinant human SAFB1 protein fragments expressed in E.coli.
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StorageMouse IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at +4°C short term. Store at -20°C long term. Avoid freeze / thaw cycle.
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PurificationAffinity-chromatography
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IsotypeMouse IgG1
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ClonalityMonoclonal
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DescriptionBinds to scaffold/matrix attachment region (S/MAR) DNA and forms a molecular assembly point to allow the formation of a 'transcriptosomal' complex (consisting of SR proteins and RNA polymerase II) coupling transcription and RNA processing By similarity. Can function as an estrogen receptor corepressor and can also bind to the HSP27 promoter and decrease its transcription.
Applications
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ReactivityHuman
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ApplicationWB,ICC/IF
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Recommended dilutionWB 1:1000 ICC/IF 1:200
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Western blot detection of SAFB1 in HelaNE,A431,MDA-MB-468,Breast cancer,ZR75-1 and MCF7 cell lysates using SAFB1 mouse mAb (1:4000 diluted).Predicted band size: 130kDa.Observed band size: 130kDa. -
Immunofluorescent analysis of HeLa cells fixed with 4% Paraformaldehyde and using SAFB1 mouse mAb (dilution 1:200).
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