DNA-PKcs Mouse mAb_单克隆抗体-上海泊湾生物技术有限公司

DNA-PKcs Mouse mAb

Source: Mouse mAb Catalog No. : AB3205

DNA-PKcs Mouse mAb (AB3205)
UniProt:P78527
Application:WB,IHC,ICC/IF,IP
Reactivity:Human
Source:Mouse mAbhttp://www.abways.cn/showproduct.asp?cid=AB3205

 Datasheet

数据表

UniProt

P78527
Mol Weight

450KDa
Alternative Names

DNA- PKcs; DNA-dependent protein kinase catalytic subunit; DNPK1; P460; PRKD; PRKDC; XRCC7; kinase DNA-PK;
Immunogen

Purified recombinant human DNA-PKcs protein fragments expressed in E.coli
Storage

Mouse IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at +4°C short term. Store at -20°C long term. Avoid freeze / thaw cycle.
Purification

Affinity-chromatography
Isotype

Mouse IgG1
Clonality

Monoclonal
Description

Serine/threonine-protein kinase that acts as a molecular sensor for DNA damage.Involved in DNA nonhomologous end joining(NHEJ)required for double-strand break(DSB)repair and V(D)J recombination.Must be bound to DNA to express its catalytic properties.Promotes processing of hairpin DNA structures in V(D)J recombination by activation of the hairpin endonuclease artemis(DCLRE1C).

Applications

Reactivity

Human
Application

WB,IHC,ICC/IF,IP
Recommended dilution

WB 1:1000 IHC 1:200 ICC/IF 1:100 IP

Western blot detection of DNA-PKcs in Hela and K562 cell lysates using DNA-PKcs mouse mAb (1:1000 diluted).Predicted band size:450KDa,Observed band size:450KDa.
Immunohistochemical analysis of paraffin-embedded Breast cancer using DNA-PKcs mouse mAb (1/200 dilution).Antigen retrieval was performed by pressure cooking in citrate buffer (pH 6.0).
Immunofluorescent analysis stain of Hela using DNA-PKcs mouse mAb (1:100).
Immunoprecipitation analysis of Hela cell lysate using DNA-PKcs mouse mAb.