DNA-PKcs Mouse mAb
Source: Mouse mAb Catalog No. : AB3206
DNA-PKcs Mouse mAb (AB3206)
UniProt:P78527
Application:WB,ICC/IF,IP
Reactivity:Human
Source:Mouse mAbhttp://www.abways.cn/showproduct.asp?cid=AB3206
数据表
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UniProt
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Mol Weight450KDa
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Alternative NamesDNA- PKcs; DNA-dependent protein kinase catalytic subunit; DNPK1; P460; PRKD; PRKDC; XRCC7; kinase DNA-PK;
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ImmunogenPurified recombinant human DNA-PKcs protein fragments expressed in E.coli
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StorageMouse IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at +4°C short term. Store at -20°C long term. Avoid freeze / thaw cycle.
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PurificationAffinity-chromatography
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IsotypeMouse lgG2b
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ClonalityMonoclonal
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DescriptionSerine/threonine-protein kinase that acts as a molecular sensor for DNA damage.Involved in DNA nonhomologous end joining(NHEJ)required for double-strand break(DSB)repair and V(D)J recombination.Must be bound to DNA to express its catalytic properties.
Applications
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ReactivityHuman
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ApplicationWB,ICC/IF,IP
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Recommended dilutionWB 1:1000 ICC/IF 1:200 IP
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Western blot detection of DNA-PKcs in Hela,K562,HL-60 and MOLT-4 cell lysates using DNA-PKcs mouse mAb (1:1000 diluted).Predicted band size:450KDa,Observed band size:450KDa. -
Immunofluorescent analysis stain of Hela using DNA-PKcs mouse mAb (1:200). -
Immunoprecipitation analysis of Hela cell lysates using DNA-PKcs mouse mAb.
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