PKM2 Mouse mAb
Source: Mouse mAb Catalog No. : AB3215
PKM2 Mouse mAb (AB3215)
UniProt:P14618
Application:WB,ICC/IF,IP
Reactivity:Human,Mouse,Monkey
Source:Mouse mAbhttp://www.abways.cn/showproduct.asp?cid=AB3215
数据表
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UniProt
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Mol Weight60KDa
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Alternative NamesCTHBP; Cytosolic thyroid hormone binding protein; KPYM; OIP 3; Oip3; OIP3; OPA interacting protein 3; Opa-interacting protein 3; p58; PK Muscle type; PK; muscle type; PK2; Pk3; PKM;
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ImmunogenPurified recombinant human PKM2 protein fragments expressed in E.coli.
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StorageMouse IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at +4°C short term. Store at -20°C long term. Avoid freeze / thaw cycle.
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PurificationAffinity-chromatography
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IsotypeMouse IgG2b
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ClonalityMonoclonal
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DescriptionGlycolytic enzyme that catalyzes the transfer of a phosphoryl group from phosphoenolpyruvate (PEP) to ADP, generating ATP. Stimulates POU5F1-mediated transcriptional activation. Plays a general role in caspase independent cell death of tumor cells.
Applications
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ReactivityHuman,Mouse,Monkey
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ApplicationWB,ICC/IF,IP
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Recommended dilutionWB 1:1000 ICC/IF 1:500 IP
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Western blot detection of PKM2 in HCT116,COS7,K562,A549 and Hela cell lysates using PKM2 mouse mAb (1:1000 diluted).Predicted band size:60KDa.Observed band size:60KDa. -
Immunofluorescent analysis of 3T3 cells fixed by anhydrous methanol for 2 h at -20℃ and using anti-PKM2 mouse mAb (dilution 1:500). -
Immunoprecipitation analysis of Hela cell lysates using PKM2.
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