PKM2 Mouse mAb
Source: Mouse mAb Catalog No. : AB3216
PKM2 Mouse mAb (AB3216)
UniProt:P14618
Application:WB,ICC/IF
Reactivity:Human,Mouse,Rat,Monkey
Source:Mouse mAbhttp://www.abways.cn/showproduct.asp?cid=AB3216
数据表
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UniProt
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Mol Weight60KDa
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Alternative NamesCTHBP; Cytosolic thyroid hormone binding protein; KPYM; OIP 3; Oip3; OIP3; OPA interacting protein 3; Opa-interacting protein 3; p58; PK Muscle type; PK; muscle type; PK2; Pk3; PKM;
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ImmunogenPurified recombinant human PKM2 protein fragments expressed in E.coli.
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StorageMouse IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at +4°C short term. Store at -20°C long term. Avoid freeze / thaw cycle.
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PurificationAffinity-chromatography
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IsotypeMouse IgG1
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ClonalityMonoclonal
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DescriptionGlycolytic enzyme that catalyzes the transfer of a phosphoryl group from phosphoenolpyruvate (PEP) to ADP, generating ATP. Stimulates POU5F1-mediated transcriptional activation. Plays a general role in caspase independent cell death of tumor cells. The ratio betwween the highly active tetrameric form and nearly inactive dimeric form determines whether glucose carbons are channeled to biosynthetic processes or used for glycolytic ATP production.
Applications
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ReactivityHuman,Mouse,Rat,Monkey
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ApplicationWB,ICC/IF
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Recommended dilutionWB 1:1000 ICC/IF 1:400
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Western blot detection of PKM2 in COS7,PC12,C6,3T3 and Hela cell lysates using PKM2 mouse mAb (1:1000 diluted).Predicted band size:60KDa.Observed band size:60KDa. -
Immunofluorescent analysis of Hela cells fixed with 4% Paraformaldehyde and using anti-PKM2 mouse mAb (dilution 1:400).
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