Pyruvate Dehydrogenase E2 Mouse mAb_单克隆抗体-上海泊湾生物技术有限公司

Pyruvate Dehydrogenase E2 Mouse mAb

Source: Mouse mAb Catalog No. : AB3221

Pyruvate Dehydrogenase E2 Mouse mAb (AB3221)
UniProt:P10515
Application:WB,ICC/IF,IP
Reactivity:Human,Mouse
Source:Mouse mAbhttp://www.abways.cn/showproduct.asp?cid=AB3221

 Datasheet

数据表

UniProt

P10515
Mol Weight

69KDa
Alternative Names

DLAT; DLAT; DLTA M2 antigen complex 70 kDa subunit; M2 Antigen Complex 70kD Subunit; PBC; PDC E2; PDC-E2; PDCE2;
Immunogen

Purified recombinant human Pyruvate Dehydrogenase E2 protein fragments expressed in E.coli.
Storage

Mouse IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at +4°C short term. Store at -20°C long term. Avoid freeze / thaw cycle.
Purification

Affinity-chromatography
Isotype

Mouse IgG1
Clonality

Monoclonal
Description

The pyruvate dehydrogenase complex catalyzes the overall conversion of pyruvate to acetyl-CoA and CO2. It contains multiple copies of three enzymatic components: pyruvate dehydrogenase (E1), dihydrolipoamide acetyltransferase (E2) and lipoamide dehydrogenase (E3).

Applications

Reactivity

Human,Mouse
Application

WB,ICC/IF,IP
Recommended dilution

WB 1:1000 ICC/IF 1:300 IP

Western blot detection of Pyruvate Dehydrogenase E2 in Jurkat,A549,U251,F9,Lncap and Hela cell lysates using Pyruvate Dehydrogenase E2 mouse mAb (1:1000 diluted).Predicted band size:69KDa.Observed band size:69KDa.
Immunofluorescent analysis stain of Hela using Pyruvate Dehydrogenase E2 mouse mAb (1:300).
Immunoprecipitation analysis of Hela cell lysates using Pyruvate Dehydrogenase E2 mouse mAb.