PCNA Mouse mAb
Source: Mouse mAb Catalog No. : AB3275
PCNA Mouse mAb (AB3275)
UniProt:P12004
Application:WB,ICC/IF,IP
Reactivity:Human,Mouse,Monkey,Rat,Hamster
Source:Mouse mAbhttp://www.abways.cn/showproduct.asp?cid=AB3275
数据表
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UniProt
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Mol Weight36KDa
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Alternative NamesCyclin; DNA polymerase delta auxiliary protein; PCNA;
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ImmunogenPurified recombinant human PCNA protein fragments expressed in E.coli.
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StorageMouse IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at +4°C short term. Store at -20°C long term. Avoid freeze / thaw cycle.
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PurificationAffinity-chromatography
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IsotypeMouse IgG2b
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ClonalityMonoclonal
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DescriptionAuxiliary protein of DNA polymerase delta and is involved in the control of eukaryotic DNA replication by increasing the polymerase's processibility during elongation of the leading strand.Induces a robust stimulatory effect on the 3'-5' exonuclease and 3'-phosphodiesterase,but not apurinic-apyrimidinic (AP) endonuclease,APEX2 activities.
Applications
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ReactivityHuman,Mouse,Monkey,Rat,Hamster
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ApplicationWB,ICC/IF,IP
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Recommended dilutionWB 1:1000 ICC/IF 1:100 IP
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Western blot detection of PCNA in Hela,3T3,COS7,C6 and CHO-K1 cell lysates using PCNA mouse mAb (1:1000 diluted).Predicted band size:36KDa.Observed band size:36KDa. -
Immunofluorescent analysis of HeLa cells using PCNA mouse mAb (dilution 1:100).Fixed in 100% methanol for 2hr at -20℃. -
Immunoprecipitation analysis of Hela cell lysates using PCNA mouse mAb.
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