WDR77 Mouse mAb_单克隆抗体-上海泊湾生物技术有限公司

WDR77 Mouse mAb

Source: Mouse mAb Catalog No. : AB3277

WDR77 Mouse mAb (AB3277)
UniProt:Q9BQA1
Application:WB,ICC/IF
Reactivity:Human,Mouse,Rat
Source:Mouse mAbhttp://www.abways.cn/showproduct.asp?cid=AB3277

 Datasheet

数据表

UniProt

Q9BQA1
Mol Weight

42KDa
Alternative Names

Androgen receptor cofactor p44; C79984; MEP50; Methylosome protein 50; p44/Mep50; RGD1310479; RP11 552M11.3; WDR77;
Immunogen

Purified recombinant human WDR77 protein fragments expressed in E.coli.
Storage

Mouse IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at +4°C short term. Store at -20°C long term. Avoid freeze / thaw cycle.
Purification

Affinity-chromatography
Isotype

Mouse IgG1
Clonality

Monoclonal
Description

Non-catalytic component of the 20S PRMT5-containing methyltransferase complex,which modifies specific arginines to dimethylarginines in several spliceosomal Sm proteins and histones.This modification targets Sm proteins to the survival of motor neurons (SMN) complex for assembly into small nuclear ribonucleoprotein core particles.Might play a role in transcription regulation.

Applications

Reactivity

Human,Mouse,Rat
Application

WB,ICC/IF
Recommended dilution

WB 1:1000 ICC/IF 1:100

Western blot detection of WDR77 in C6,3T3 and K562 cell lysates using WDR77 mouse mAb (1:1000 diluted).Predicted band size:42KDa.Observed band size:42KDa.
Immunofluorescent analysis of HeLa cells fixed in 1% Paraformaldehyde and then permeabilized in 0.1% Triton X-100,next using WDR77 mouse mAb (dilution 1:100).