RPA32/RPA2 Mouse mAb_单克隆抗体-上海泊湾生物技术有限公司

RPA32/RPA2 Mouse mAb

Source: Mouse mAb Catalog No. : AB3281

RPA32/RPA2 Mouse mAb (AB3281)
UniProt:P15927
Application:WB,ICC/IF,IP
Reactivity:Human
Source:Mouse mAbhttp://www.abways.cn/showproduct.asp?cid=AB3281

 Datasheet

数据表

UniProt

P15927
Mol Weight

32KDa
Alternative Names

REPA2; RF-A; RP-A; RPA2; RPA32; replication factor-A protein 2;
Immunogen

Purified recombinant human RPA32/RPA2 protein fragments expressed in E.coli.
Storage

Mouse IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at +4°C short term. Store at -20°C long term. Avoid freeze / thaw cycle.
Purification

Affinity-chromatography
Isotype

Mouse IgG2b
Clonality

Monoclonal
Description

Required for DNA recombination,repair and replication.The activity of RP-A is mediated by single-stranded DNA binding and protein interactions.Required for the efficient recruitment of the DNA double-strand break repair factor RAD51 to chromatin in response to DNA damage.Functions as component of the alternative replication protein A complex (aRPA).

Applications

Reactivity

Human
Application

WB,ICC/IF,IP
Recommended dilution

WB 1:2000 ICC/IF 1:200 IP

Western blot detection of RPA32/RPA2 in MCF7,Hela and HUVEC cell lysates using RPA32/RPA2 mouse mAb (1:2000 diluted).Predicted band size:32KDa.Observed band size:32KDa.Exposure time:20s.
Immunofluorescent analysis of HeLa cells fixed with -20℃ Methanol and using anti-RPA32/RPA2 antibody (dilution 1:200).
Immunoprecipitation analysis of Hela cell lysates using RPA32/RPA2 mouse mAb.