ZAP-70 Mouse mAb
Source: Mouse mAb Catalog No. : AB3285
ZAP-70 Mouse mAb (AB3285)
UniProt:P43403
Application:WB,IP
Reactivity:Human
Source:Mouse mAbhttp://www.abways.cn/showproduct.asp?cid=AB3285
数据表
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UniProt
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Mol Weight70KDa
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Alternative NamesTyrosine-protein kinase ZAP-70; ZAP70; SRK;
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ImmunogenPurified recombinant human ZAP-70 protein fragments expressed in E.coli.
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StorageMouse IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at +4°C short term. Store at -20°C long term. Avoid freeze / thaw cycle.
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PurificationAffinity-chromatography
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IsotypeMouse IgG2b
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ClonalityMonoclonal
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DescriptionTyrosine kinase that plays an essential role in regulation of the adaptive immune response.Regulates motility,adhesion and cytokine expression of mature T-cells,as well as thymocyte development.Contributes also to the development and activation of primary B-lymphocytes.When antigen presenting cells (APC) activate T-cell receptor (TCR),a serie of phosphorylations lead to the recruitment of ZAP70 to the doubly phosphorylated TCR component CD247/CD3Z through ITAM motif at the plasma membrane.
Applications
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ReactivityHuman
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ApplicationWB,IP
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Recommended dilutionWB 1:1000 IP
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Western blot detection of ZAP-70 in CEM and Jurkat cell lysates,negative in the Ramos cell lysates using ZAP-70 mouse mAb (1:1000 diluted).Predicted band size:70KDa.Observed band size:70KDa. -
Immunoprecipitation analysis of Jurkat cell lysates (ZAP-70 positive expression cell line)and Ramos cell lysates(ZAP-70 negative expression cell line) using ZAP-70 mouse mAb.
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