hHR23b Mouse mAb
Source: Mouse mAb Catalog No. : AB3287
hHR23b Mouse mAb (AB3287)
UniProt:P54727
Application:WB,IHC,ICC/IF
Reactivity:Human,Mouse,Rat,Monkey,Hamster
Source:Mouse mAbhttp://www.abways.cn/showproduct.asp?cid=AB3287
数据表
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UniProt
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Mol Weight58KDa
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Alternative NamesUV excision repair protein RAD23 homolog B; hHR23B; p58; RAD23B;
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ImmunogenPurified recombinant human hHR23b protein fragments expressed in E.coli.
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StorageMouse IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at +4°C short term. Store at -20°C long term. Avoid freeze / thaw cycle.
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PurificationAffinity-chromatography
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IsotypeMouse IgG2b
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ClonalityMonoclonal
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DescriptionMultiubiquitin chain receptor involved in modulation of proteasomal degradation. Binds to polyubiquitin chains. Proposed to be capable to bind simultaneously to the 26S proteasome and to polyubiquitinated substrates and to deliver ubiquitinated proteins to the proteasome. May play a role in endoplasmic reticulum-associated degradation (ERAD) of misfolded glycoproteins by association with PNGase and delivering deglycosylated proteins to the proteasome.
Applications
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ReactivityHuman,Mouse,Rat,Monkey,Hamster
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ApplicationWB,IHC,ICC/IF
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Recommended dilutionWB 1:1000 IHC 1:100 ICC/IF 1:100
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Western blot detection of hHR23b in A431,K562,Jurkat,C6,3T3 and Hela cell lysates using hHR23b mouse mAb (1:1000 diluted).Predicted band size:58KDa.Observed band size:58KDa.Exposure time:5min. -
Immunohistochemical analysis of paraffin-embedded Prostate Cancer using hHR23b mouse mAb (1/100 dilution).Antigen retrieval was performed by pressure cooking in citrate buffer (pH 6.0). -
Immunofluorescent analysis of HeLa cells fixed with 4% Paraformaldehyde and using anti-hHR23b antibody (dilution 1:100).
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