Ku70 Mouse mAb_单克隆抗体-上海泊湾生物技术有限公司

Ku70 Mouse mAb

Source: Mouse mAb Catalog No. : AB3293

Ku70 Mouse mAb (AB3293)
UniProt:P12956
Application:WB,ICC/IF,IP
Reactivity:Human,Monkey
Source:Mouse mAbhttp://www.abways.cn/showproduct.asp?cid=AB3293

 Datasheet

数据表

UniProt

P12956
Mol Weight

67KDa
Alternative Names

CTC75; CTCBF; DNA repair protein XRCC6; G22P1; Ku 70; Ku autoantigen 70kDa; Ku autoantigen, 70kDa; Ku p70; Ku70; Kup70; TLAA; XRCC6;
Immunogen

Purified recombinant human Ku70 protein fragments expressed in E.coli.
Storage

Mouse IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at +4°C short term. Store at -20°C long term. Avoid freeze / thaw cycle.
Purification

Affinity-chromatography
Isotype

Mouse IgG1
Clonality

Monoclonal
Description

Has a role in chromosome translocation. The DNA helicase II complex binds preferentially to fork-like ends of double-stranded DNA in a cell cycle-dependent manner. It works in the 3'-5' direction. Binding to DNA may be mediated by XRCC6. Involved in DNA non-homologous end joining (NHEJ) required for double-strand break repair and V(D)J recombination.

Applications

Reactivity

Human,Monkey
Application

WB,ICC/IF,IP
Recommended dilution

WB 1:1000 ICC/IF 1:200 IP

Western blot detection of Ku70 in Hela,A549,COS7 and K562 cell lysates using Ku70 mouse mAb (1:1000 diluted).Predicted band size:70KDa.Observed band size:67KDa.
Immunofluorescent analysis of HeLa cells fixed with -20℃ Methanol and using anti-Ku70 antibody (dilution 1:200).
Immunoprecipitation analysis of Hela cell lysates using Ku70 mouse mAb.