FEN-1 Mouse mAb
Source: Mouse mAb Catalog No. : AB3306
FEN-1 Mouse mAb (AB3306)
UniProt:P39748
Application:WB,ICC/IF
Reactivity:Human,Mouse,Rat,Monkey,Hamster
Source:Mouse mAbhttp://www.abways.cn/showproduct.asp?cid=AB3306
数据表
-
UniProt
-
Mol Weight45KDa
-
Alternative NamesDNase IV; FEN1; Flap endonuclease 1; Flap structure specific endonuclease 1; hFEN-1; hFEN1; Maturation factor 1; MF1; Rad2;
-
ImmunogenPurified recombinant human FEN-1 protein fragments expressed in E.coli.
-
StorageMouse IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at +4°C short term. Store at -20°C long term. Avoid freeze / thaw cycle.
-
PurificationAffinity-chromatography
-
IsotypeMouse IgG1
-
ClonalityMonoclonal
-
DescriptionStructure-specific nuclease with 5'-flap endonuclease and 5'-3' exonuclease activities involved in DNA replication and repair.During DNA replication,cleaves the 5'-overhanging flap structure that is generated by displacement synthesis when DNA polymerase encounters the 5'-end of a downstream Okazaki fragment.It enters the flap from the 5'-end and then tracks to cleave the flap base,leaving a nick for ligation.
Applications
-
ReactivityHuman,Mouse,Rat,Monkey,Hamster
-
ApplicationWB,ICC/IF
-
Recommended dilutionWB 1:1000 ICC/IF 1:400
-
Western blot detection of FEN-1 in Hela,Jurkat,3T3,COS7,PC-12,C6,Raw264.7,Raji and CHO-K1 cell lysates using FEN-1 mouse mAb (1:1000 diluted).Predicted band size:45KDa.Observed band size:45KDa. -
Immunofluorescent analysis of HeLa cells fixed with 4% Paraformaldehyde and using FEN-1 mouse mAb (dilution 1:400).
销售咨询
一键电话