FEN-1 Mouse mAb
Source: Mouse mAb Catalog No. : AB3307
FEN-1 Mouse mAb (AB3307)
UniProt:P39748
Application:WB,IP
Reactivity:Human,Mouse,Rat
Source:Mouse mAbhttp://www.abways.cn/showproduct.asp?cid=AB3307
数据表
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UniProt
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Mol Weight45KDa
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Alternative NamesDNase IV; FEN1; Flap endonuclease 1; Flap structure specific endonuclease 1; hFEN-1; hFEN1; Maturation factor 1; MF1; Rad2;
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ImmunogenPurified recombinant human FEN-1 protein fragments expressed in E.coli.
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StorageMouse IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at +4°C short term. Store at -20°C long term. Avoid freeze / thaw cycle.
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PurificationAffinity-chromatography
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IsotypeMouse IgG1
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ClonalityMonoclonal
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DescriptionStructure-specific nuclease with 5'-flap endonuclease and 5'-3' exonuclease activities involved in DNA replication and repair.During DNA replication,cleaves the 5'-overhanging flap structure that is generated by displacement synthesis when DNA polymerase encounters the 5'-end of a downstream Okazaki fragment.It enters the flap from the 5'-end and then tracks to cleave the flap base,leaving a nick for ligation.
Applications
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ReactivityHuman,Mouse,Rat
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ApplicationWB,IP
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Recommended dilutionWB 1:1000 IP
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WWestern blot detection of FEN-1 in Hela,Hela NE,Jurkat,K562 and 3T3 cell lysates using FEN-1 mouse mAb (1:1000 diluted).Predicted band size:45KDa.Observed band size:45KDa. -
Immunoprecipitation analysis of Hela cell lysates using FEN-1 mouse mAb.
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