DDX3 Mouse mAb
Source: Mouse mAb Catalog No. : AB3308
DDX3 Mouse mAb (AB3308)
UniProt:O00571
Application:WB,ICC/IF,IP
Reactivity:Human,Rat,Mouse,Monkey
Source:Mouse mAbhttp://www.abways.cn/showproduct.asp?cid=AB3308
数据表
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UniProt
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Mol Weight75KDa
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Alternative NamesDBX; DDX3; HLP2; DDX14;
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ImmunogenPurified recombinant human DDX3 protein fragments expressed in E.coli.
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StorageMouse IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at +4°C short term. Store at -20°C long term. Avoid freeze / thaw cycle.
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PurificationAffinity-chromatography
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IsotypeMouse IgG2a
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ClonalityMonoclonal
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DescriptionMultifunctional ATP-dependent RNA helicase.The ATPase activity can be stimulated by various ribo- and deoxynucleic acids indicative for a relaxed substrate specificity.In vitro can unwind partially double-stranded DNA with a preference for 5'-single-stranded DNA overhangs.
Applications
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ReactivityHuman,Rat,Mouse,Monkey
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ApplicationWB,ICC/IF,IP
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Recommended dilutionWB 1:2000 ICC/IF 1:200 IP
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Western blot detection of DDX3 in Hela,3T3,C6,COS7,K562 and Jurkat cell lysate using DDX3 mouse mAb (1:1000 diluted).Predicted band size: 75KDa.Observed band size: 75KDa. -
Immunofluorescent analysis of HeLa cells fixed with 4% Paraformaldehyde and using DDX3 mouse mAb (dilution 1:200). -
Immunoprecipitation analysis of Hela cell lysates using DDX3 mouse mAb.
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