NPTII Mouse mAb
Source: Mouse mAb Catalog No. : AB3319
NPTII Mouse mAb (AB3319)
UniProt:
Application:WB
Reactivity:recombinant protein
Source:Mouse mAbhttp://www.abways.cn/showproduct.asp?cid=AB3319
数据表
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UniProt
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Mol Weight29KDa
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Alternative NamesAminoglycoside 3' phosphotransferase; APH(3')II; aphA; Neomycine phosphotransferase; NPTII;
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Immunogen
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StorageMouse IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at +4°C short term. Store at -20°C long term. Avoid freeze / thaw cycle.
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PurificationAffinity-chromatography
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IsotypeMouse IgG1
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ClonalityMonoclonal
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DescriptionNeomycin phosphotransferase II confers resistance to kanamycin and neomycin in bacteria and G418 (Geneticin®, G418 Sulfate) in mammalian cells. These antibiotics bind to ribosomal components and inhibit protein synthesis. NPT II inhibits these antibiotics through phosphorylation, which is thought to interfere with their active transport into the cell.
Applications
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Reactivityrecombinant protein
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ApplicationWB
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Recommended dilutionWB 1:1000
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Western blot detection of NPTⅡ in non-trangenic Brassica napus L. and transgenic Brassica napus L. cell lysates using NPTⅡ mouse mAb (1:1000 diluted).Predicted band size:29KDa.Observed band size:29KDa.
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