E2F-1 Mouse mAb_单克隆抗体-上海泊湾生物技术有限公司

E2F-1 Mouse mAb

Source: Mouse mAb Catalog No. : AB3321

E2F-1 Mouse mAb (AB3321)
UniProt:Q01094
Application:WB,ICC/IF,IP
Reactivity:Human,Rat
Source:Mouse mAbhttp://www.abways.cn/showproduct.asp?cid=AB3321

数据表

UniProt

Q01094
Mol Weight

70KDa
Alternative Names

E2F-1; PBR3; PRB-binding protein E2F-1; RBAP-1; RBBP-3; RBBP3; Retinoblastoma binding protein 3; Retinoblastoma-associated protein 1; Transcription factor E2F1;
Immunogen

Purified recombinant human E2F-1 protein fragments expressed in E.coli.
Storage

Mouse IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at +4°C short term. Store at -20°C long term. Avoid freeze / thaw cycle.
Purification

Affinity-chromatography
Isotype

Mouse IgG2b
Clonality

Monoclonal
Description

Transcription activator that binds DNA cooperatively with dp proteins through the E2 recognition site, 5'-TTTC[CG]CGC-3' found in the promoter region of a number of genes whose products are involved in cell cycle regulation or in DNA replication.

Applications

Western blot detection of E2F-1 in C6,Raw264.7 and Hela cell lysates using E2F-1 mouse mAb (1:500 diluted).Predicted band size:70KDa.Observed band size:70KDa.
Immunofluorescent analysis of Hela cells fixed with 4% Paraformaldehyde and using anti-E2F-1 mouse mAb (dilution 1:100). DAPI was used to stain nucleus(blue).
Immunoprecipitation analysis of Hela cell lysates using E2F-1 mouse mAb.