eIF2 alpha Mouse mAb_单克隆抗体-上海泊湾生物技术有限公司

eIF2 alpha Mouse mAb

Source: Mouse mAb Catalog No. : AB3335

eIF2 alpha Mouse mAb (AB3335)
UniProt:P05198
Application:WB,ICC/IF,IP
Reactivity:Human,Mouse,Rat
Source:Mouse mAb
References（1）
http://www.abways.cn/showproduct.asp?cid=AB3335

 Datasheet

数据表

UniProt

P05198
Mol Weight

38KDa
Alternative Names

EIF-2; eIF-2-alpha; eIF-2A; eIF-2alpha; EIF2; EIF2A; EIF2S1; IF2A;
Immunogen

Purified recombinant human eIF2α protein fragments expressed in E.coli.
Storage

Mouse IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at +4°C short term. Store at -20°C long term. Avoid freeze / thaw cycle.
Purification

Affinity-chromatography
Isotype

Mouse IgG2b
Clonality

Monoclonal
Description

Functions in the early steps of protein synthesis by forming a ternary complex with GTP and initiator tRNA. This complex binds to a 40S ribosomal subunit, followed by mRNA binding to form a 43S preinitiation complex.

Applications

Reactivity

Human,Mouse,Rat
Application

WB,ICC/IF,IP
Recommended dilution

WB 1:1000 ICC/IF 1:200 IP

Western blot detection of eIF2α in K562,COS7,3T3, C2C12,MCF-7,C6 and Hela cell lysates using eIF2α mouse mAb (1:1000 diluted).Predicted band size:38KDa.Observed band size:38KDa.
Immunofluorescent analysis of Hela cells fixed by anhydrous methanol for 2 h at -20℃ and using anti-eIF2α mouse mAb (dilution 1:200).
Immunoprecipitation analysis of Hela cell lysates using eIF2α.

引用文献（1条）

Induction of the Unfolded Protein Response during Bovine Alphaherpesvirus 1 Infection-viruses (Viruses-Basel VIRUSES-BASEL)