Vimentin Mouse mAb
Source: Mouse mAb Catalog No. : AB3339
Vimentin Mouse mAb (AB3339)
UniProt:P08670
Application:WB,ICC/IF,IP
Reactivity:Human
Source:Mouse mAbhttp://www.abways.cn/showproduct.asp?cid=AB3339
数据表
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UniProt
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Mol Weight57KDa
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Alternative NamesCTRCT30; Epididymis luminal protein 113; HEL113; VIM; Vimentin.
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ImmunogenPurified recombinant human Vimentin protein fragments expressed in E.coli.
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StorageMouse IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at +4°C short term. Store at -20°C long term. Avoid freeze / thaw cycle.
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PurificationAffinity-chromatography
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IsotypeMouse IgG2a
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ClonalityMonoclonal
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DescriptionVimentins are class-III intermediate filaments found in various non-epithelial cells, especially mesenchymal cells. Vimentin is attached to the nucleus, endoplasmic reticulum, and mitochondria, either laterally or terminally.
Applications
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ReactivityHuman
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ApplicationWB,ICC/IF,IP
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Recommended dilutionWB 1:1000 ICC/IF 1:800 IP
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Western blot detection of Vimentin in Molt-4,K562,COS7,Jurkat,Hela and Vimentin negative cell (Daudi,Ramos,Raji) lysates using Vimentin mouse mAb (1:1000 diluted).Predicted band size:57KDa.Observed band size:57KDa. -
Immunofluorescent analysis of Hela cells fixed with 4% Paraformaldehyde and using anti-Vimentin mouse mAb (dilution 1:800). -
Immunoprecipitation analysis of Hela cell lysates using Vimentin mouse mAb.
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