CD44 (2H12) Mouse mAb_单克隆抗体-上海泊湾生物技术有限公司

CD44 (2H12) Mouse mAb

Source: Mouse mAb Catalog No. : AB3395

CD44 (2H12) Mouse mAb (AB3395)
UniProt:P16070
Application:WB,IHC,ICC/IF,IP
Reactivity:Human
Source:Mouse mAbhttp://www.abways.cn/showproduct.asp?cid=AB3395

 Datasheet

数据表

UniProt

P16070
Mol Weight

80kDa
Alternative Names

IN; LHR; MC56; MDU2; MDU3; MIC4; Pgp1; CDW44; CSPG8; HCELL; HUTCH-I; ECMR-III; CD44
Immunogen

Purified recombinant human CD44 protein fragments expressed in E.coli.
Storage

Mouse IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at +4°C short term. Store at -20°C long term. Avoid freeze / thaw cycle.
Purification

Affinity-chromatography
Isotype

Mouse IgG1
Clonality

Monoclonal
Description

Swiss-Prot Acc.P16070.Receptor for hyaluronic acid (HA). Mediates cell-cell and cell-matrix interactions through its affinity for HA, and possibly also through its affinity for other ligands such as osteopontin, collagens, and matrix metalloproteinases (MMPs). Adhesion with HA plays an important role in cell migration, tumor growth and progression. Also involved in lymphocyte activation, recirculation and homing, and in hematopoiesis. Altered expression or dysfunction causes numerous pathogenic phenotypes. Great protein heterogeneity due to numerous alternative splicing and post-translational modification events.

Applications

Reactivity

Human
Application

WB,IHC,ICC/IF,IP
Recommended dilution

WB 1:500-1:1000 IHC 1:50-1:100 ICC/IF 1:50-1:200 IP 1:20

Western blot detection of CD44 in HUVEC cell lysates using CD44 mouse mAb(dilution 1:1000).Predicted band size:82kDa.Observed band size:80kDa.
Immunohistochemical analysis of paraffin-embedded human tonsils using CD44 (2H12) Mouse mAb.
Immunofluorescent analysis of HeLa cells fixed with 1% Paraformaldehyde and using CD44 Mouse mAb (dilution 1:200,green). DAPI was used to stain nucleus(blue).
Immunoprecipitation analysis of Hela cell lysates using CD44 mouse mAb.