eIF4E (8C8) Mouse mAb
Source: Mouse mAb Catalog No. : AB3399
eIF4E (8C8) Mouse mAb (AB3399)
UniProt:P06730
Application:WB
Reactivity:Human,Mouse,Rat
Source:Mouse mAbhttp://www.abways.cn/showproduct.asp?cid=AB3399
数据表
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UniProt
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Mol Weight25kDa
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Alternative NamesCBP; EIF4F; AUTS19; EIF4E1; eIF-4E; EIF4EL1; EIF4E
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ImmunogenPurified recombinant human eif4e protein expressed in E.coli.
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StorageMouse IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at +4°C short term. Store at -20°C long term. Avoid freeze / thaw cycle.
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PurificationAffinity-chromatography
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IsotypeMouse IgG1
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ClonalityMonoclonal
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DescriptionSwiss-Prot Acc.P06730.Its translation stimulation activity is repressed by binding to the complex CYFIP1-FMR1. Recognizes and binds the 7-methylguanosine-containing mRNA cap during an early step in the initiation of protein synthesis and facilitates ribosome binding by inducing the unwinding of the mRNAs secondary structures. Component of the CYFIP1-EIF4E-FMR1 complex which binds to the mRNA cap and mediates translational repression. In the CYFIP1-EIF4E-FMR1 complex this subunit mediates the binding to the mRNA cap.
Applications
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ReactivityHuman,Mouse,Rat
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ApplicationWB
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Recommended dilutionWB 1:500-1:1000
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Western blot detection of eIF4E in MDA-MB-468, ZR75-1, MCF7, T47D and CHO-K1 cell lysates using eIF4E mouse mAb (1:1000 diluted). Predicted band size: 25KDa. Observed band size:25KDa. -
Western blot detection of eIF4E in Jurkat, Hela, MCF7, K562, C6, 3T3, PC12, HepG2, CHO-K1, Raji, Raw264.7 and Ramos cell lysates using eIF4E mouse mAb (1:2000 diluted). Predicted band size: 25KDa. Observed band size:25KDa.
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