P40692

85kDa

FCC2; COCA2; HNPCC

Purified recombinant fragment of MLH1 (aa381-483) expressed in E. Coli.

Mouse IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at +4°C short term. Store at -20°C long term. Avoid freeze / thaw cycle.

Affinity-chromatography

Mouse IgG1

Monoclonal

Swiss-Prot Acc.P40692.DNA-mismatch repair (MMR), a conserved process that involves correcting errors made during DNA synthesis, is crucial to the maintenance of genomic integrity. Lack of a functional DNA-mismatch repair pathway is a commoncharacteristic of several different types of human cancers, either due to anMMR gene mutation or promoter-methylation gene silencing. MLH1 is a human homolog of the E. coli DNA mismatch repair gene mutL, consistent with the characteristic alterations in microsatellite sequences (RER+ phenotype) found in hereditary nonpolyposis colon cancer (HNPCC). MLH1 is an integralpart of the protein complex responsible for mismatch repair expressed inlymphocytes, heart, colon, breast, lung, spleen, testis, prostate, thyroid andgall bladder, and is methylated in several ovarian tumors. Loss of MLH1 protein expression is associated with a mutated phenotype, microsatellite instability and a predisposition to cancer. In hereditary nonpolyposis colorectalcancer (HNPCC), an autosomal dominant inherited cancer syndrome that signifies a high risk of colorectal and various other types of cancer, the MLH1 gene exhibits a pathogenic mutation. Inactivation of the MLH1 gene causes genome instability and predisposition to cancer. MLH1 also plays a role in meiotic recombination.

Human,Monkey

WB,ICC/IF

WB 1:500-1:1000 ICC/IF 1:50-1:200