LPP (6F6) Mouse mAb
Source: Mouse mAb Catalog No. : AB3429
LPP (6F6) Mouse mAb (AB3429)
UniProt:Q93052
Application:WB,IHC,ICC/IF,IP
Reactivity:Human,Mouse,Monkey,Hamster
Source:Mouse mAbhttp://www.abways.cn/showproduct.asp?cid=AB3429
数据表
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UniProt
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Mol Weight66kDa
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Alternative NamesLPP
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ImmunogenPurified recombinant fragment of human LPP expressed in E. Coli.
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StorageMouse IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at +4°C short term. Store at -20°C long term. Avoid freeze / thaw cycle.
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PurificationAffinity-chromatography
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IsotypeMouse IgG1
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ClonalityMonoclonal
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DescriptionSwiss-Prot Acc.Q93052.LIM domain containing preferred translocation partner in lipoma. The Zyxin family of proteins contains five members, Ajuba, LIMD1, LPP,TRIP6 and Zyxin. LPP (LIM-containing lipoma-preferred partner), a LIM domain-containing scaffolding protein contains three LIM domains at its carboxyterminus, which are preceded by a proline-rich pre-LIM region containing a number of protein interaction domains. LPP, an 80 kDa protein, localizes to sites of cell adhesion, such as focal adhesions and cell-cell contacts,and shuttles to the nucleus where it has transcriptional activation capacity.The human LPP gene maps to chromosomal location 3q28, and preferentiallytranslocates to the HMGIC gene in a subclass of human benign mesenchymal tumors known as lipomas.
Applications
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ReactivityHuman,Mouse,Monkey,Hamster
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ApplicationWB,IHC,ICC/IF,IP
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Recommended dilutionWB: 1/500-1/1000 IHC: 1/50-1/100 ICC/IF: 1/50-1/200 IP: 1/20
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Western blot detection of LPP in A549,MCF7,C6 and Hela cell lysates using LPP mouse mAb (1:5000 diluted).Predicted band size:66KDa.Observed band size:75KDa. -
Immunohistochemical analysis of paraffin-embedded human small intestine using LPP mouse mAb with DAB staining. -
Confocal immunofluorescence analysis of Hela cells using LPP mouse mAb (green). Red: Actin filaments have been labeled using DY-554 phalloidin. Blue: DRAQ5 fluorescent DNA dye. -
Immunoprecipitation analysis of Hela cell lysates using LPP (6F6) Mouse mAb.
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