Cytokeratin 18 (6A8) Mouse mAb
Source: Mouse mAb Catalog No. : AB3433
Cytokeratin 18 (6A8) Mouse mAb (AB3433)
UniProt:P05783
Application:WB,IHC
Reactivity:Human
Source:Mouse mAbhttp://www.abways.cn/showproduct.asp?cid=AB3433
数据表
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UniProt
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Mol Weight48kDa
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Alternative NamesCK18; CYK18; KRT18
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ImmunogenPurified recombinant fragment of human Cytokeratin 18 (aa391-483) expressed in E. Coli.
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StorageMouse IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at +4°C short term. Store at -20°C long term. Avoid freeze / thaw cycle.
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PurificationAffinity-chromatography
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IsotypeMouse IgG2b
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ClonalityMonoclonal
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DescriptionSwiss-Prot Acc.P05783.Cytokeratin 18, also known as CK18, CYK18, KRT18. Entrez Protein NP_000215. It encodes the type I intermediate filament chain keratin 18. Keratin 18, together with its filament partner keratin 8, are perhaps the most commonly found members of the intermediate filament gene family. They are expressed in single layer epithelial tissues of the body. Mutations in this gene have been linked to cryptogenic cirrhosis. Two transcript variants encoding the same protein have been found for this gene.
Applications
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ReactivityHuman
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ApplicationWB,IHC
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Recommended dilutionWB: 1/500-1/1000 IHC: 1/50-1/100
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Western blot detection of Cytokeratin 18 in A549 and Hela cell lysates using Cytokeratin 18 mouse mAb (1:1000 diluted).Predicted band size:48KDa.Observed band size:48KDa. -
Immunohistochemical analysis of paraffin-embedded human breast carcinoma (A), hepatocarcinoma (B), stomach cancer (C) and colon cancer tissue (D), showing cytoplasmic location with DAB staining using CK18 mouse mAb.
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