TriMethyl-Histone H3 ( Lys79) (6A6) Mouse mAb
Source: Mouse mAb Catalog No. : AB3448
TriMethyl-Histone H3 ( Lys79) (6A6) Mouse mAb (AB3448)
UniProt:P68431
Application:WB,IHC,ICC/IF,IP
Reactivity:Human,Mouse,Rat
Source:Mouse mAbhttp://www.abways.cn/showproduct.asp?cid=AB3448
数据表
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UniProt
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Mol Weight15kDa
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Alternative NamesH3 histone, HIST1H3A, Histone cluster 1, H3a
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ImmunogenSynthetic Peptide of Histone H3 (Tri Methyl Lys79)
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StorageMouse IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at +4°C short term. Store at -20°C long term. Avoid freeze / thaw cycle.
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PurificationAffinity-chromatography
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IsotypeMouse IgG1
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ClonalityMonoclonal
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DescriptionSwiss-Prot Acc.P68431.Core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling.MiscellaneousThis histone is only present in mammals and is enriched in acetylation of Lys-15 and dimethylation of Lys-10 (H3K9me2).
Applications
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ReactivityHuman,Mouse,Rat
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ApplicationWB,IHC,ICC/IF,IP
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Recommended dilutionWB: 1/500-1/2000 IHC: 1/50-1/100 ICC/IF: 1/50-1/200 IP: 1/200
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Western blot analysis of extracts from T47D,COS7 and C6 cell lines using Tri-Methyl-Histone H3 (Lys79) Mouse mAb (1:1000 diluted).Predicted band size:17KDa.Observed band size:17KDa. -
Western blot analysis of 1) Hela, 2) Rat Testis tissue, 3) Raw264.7 with Histone H3 (tri methyl K79) (3G3) Mouse MAb diluted at 1:2000. -
1) Input: Hela Cell Lysate 2) IP product: IP dilute 1:200
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