Nanog (6F5) Mouse mAb
Source: Mouse mAb Catalog No. : AB3494
Nanog (6F5) Mouse mAb (AB3494)
UniProt:Q9H9S0
Application:WB,ICC/IF
Reactivity:Human
Source:Mouse mAbhttp://www.abways.cn/showproduct.asp?cid=AB3494
数据表
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UniProt
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Mol Weight35kDa
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Alternative NamesNANOG; Homeobox protein NANOG; Homeobox transcription factor Nanog; hNanog
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ImmunogenPurified recombinant fragment of NANOG (aa20-166) expressed in E. Coli.
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StorageMouse IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at +4°C short term. Store at -20°C long term. Avoid freeze / thaw cycle.
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PurificationAffinity-chromatography
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IsotypeMouse IgG1
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ClonalityMonoclonal
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DescriptionSwiss-Prot Acc.Q9H9S0.NANOG: Nanog homeobox. Entrez Protein NP_079141. Nanog is a divergent homeodomain protein that directs pluripotency and differentiation of undifferentiated embryonic stem cells. Nanog mRNA is present in pluripotent mouse and human cell lines, and absent from differentiated cells. Human Nanog protein shares 52% overall amino acid identity with the mouse protein and 85% identity in the homeodomain. Human Nanog maps to gene locus 12p13.31, whereas mouse Nanog maps to gene loci 6 F2. Murine embryonic Nanog expression is detected in the inner cell mass of the blastocyst. High levels of human Nanog expression were detected by Northern analysis in the undifferentiated N-Tera embryonal carcinoma cell line.
Applications
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ReactivityHuman
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ApplicationWB,ICC/IF
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Recommended dilutionWB: 1/500-1/1000 ICC/IF: 1/50-1/200
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Western blot analysis of Nanog (6F5) in NTERA2 lysates using Nanog (6F5) antibody. -
Immunofluorescence analysis of Nanog (6F5) in NTERA2 cells (left) and HeLa cells (right) using Nanog antibody (green). Red: Actin filaments have been labeled with DY554 phalloidin.
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