PLK1 (1F8) Mouse mAb_单克隆抗体-上海泊湾生物技术有限公司

PLK1 (1F8) Mouse mAb

Source: Mouse mAb Catalog No. : AB3501

PLK1 (1F8) Mouse mAb (AB3501)
UniProt:P53350
Application:WB,IHC,FC
Reactivity:Human,Mouse
Source:Mouse mAbhttp://www.abways.cn/showproduct.asp?cid=AB3501

 Datasheet

数据表

UniProt

P53350
Mol Weight

68kDa
Alternative Names

PLK1; PLK; Serine/threonine-protein kinase PLK1; Polo-like kinase 1; PLK-1; Serine/threonine-protein kinase 13; STPK13
Immunogen

Purified recombinant fragment of human PLK1 (AA: 331-508) expressed in E. Coli.
Storage

Mouse IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at +4°C short term. Store at -20°C long term. Avoid freeze / thaw cycle.
Purification

Affinity-chromatography
Isotype

Mouse IgG1
Clonality

Monoclonal
Description

Swiss-Prot Acc.P53350.Polo-like kinase 1 (Plk1) plays key roles in many aspects of mitosis. Suppression of Plk1 by p21(Waf1) is responsible for p53-dependent protection against adriamycin-induced caspase-independent mitotic death.

Applications

Reactivity

Human,Mouse
Application

WB,IHC,FC
Recommended dilution

WB: 1/500-1/1000 IHC: 1/50-1/100 FC: 1/50-1/100

Western blot analysis of PLK1 in HCT116 and Jurkat lysates using PLK1 antibody.
Immunohistochemistry analysis of paraffinembedded stomach cancer tissues using PLK1 antibody with DAB staining.
Western blot analysis of PLK1 (1F8) in K562 and Raji lysates using PLK1 (1F8) antibody.
Immunohistochemistry analysis of paraffinembedded rectum cancer tissues using PLK1 (1F8) antibody.
Flow cytometry analysis of NIH3T3 cells using PLK1 antibody(green) and negative control (red).