DiMethyl-Histone H3 (Lys27) (5F6) Mouse mAb_单克隆抗体-上海泊湾生物技术有限公司

DiMethyl-Histone H3 (Lys27) (5F6) Mouse mAb

Source: Mouse mAb Catalog No. : AB3525

DiMethyl-Histone H3 (Lys27) (5F6) Mouse mAb (AB3525)
UniProt:P68431
Application:WB,ICC/IF,IP
Reactivity:Human,Mouse,Rat
Source:Mouse mAbhttp://www.abways.cn/showproduct.asp?cid=AB3525

 Datasheet

数据表

UniProt

P68431
Mol Weight

15kDa
Alternative Names

H3K27me2; H3 histone; HIST1H3A; Histone cluster 1; H3a
Immunogen

Synthetic Peptide of Histone H3 (Di Methyl Lys27)
Storage

Mouse IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at +4°C short term. Store at -20°C long term. Avoid freeze / thaw cycle.
Purification

Affinity-chromatography
Isotype

Mouse IgG1
Clonality

Monoclonal
Description

Swiss-Prot Acc.P68431.Core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling.MiscellaneousThis histone is only present in mammals and is enriched in acetylation of Lys-15 and dimethylation of Lys-10 (H3K9me2).

Applications

Reactivity

Human,Mouse,Rat
Application

WB,ICC/IF,IP
Recommended dilution

WB: 1/500-1/1000 ICC/IF: 1/50-1/200 IP: 1/20

Western blot analysis of DiMethylHistone H3 (Lys27) (5F6) in C6,3T3,C2C12 lysates using DiMethylHistone H3 (Lys27) (5F6) antibody.
Immunocytochemistry analysis ofDiMethylHistone H3 (Lys27) in HeLa cells using DiMethylHistone H3 (Lys27) (5F6) antibody.
Immunoprecipitation analysis of DiMethylHistone H3 (Lys27) (5F6) in Hela lysates using DiMethylHistone H3 (Lys27) (5F6) antibody.