eIF4A1 (6D7) Mouse mAb
Source: Mouse mAb Catalog No. : AB3532
eIF4A1 (6D7) Mouse mAb (AB3532)
UniProt:P60842
Application:WB,IHC,ICC/IF
Reactivity:Human,Mouse,Rat
Source:Mouse mAbhttp://www.abways.cn/showproduct.asp?cid=AB3532
数据表
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UniProt
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Mol Weight48kDa
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Alternative NamesEukaryotic initiation factor 4A-I; eIF-4A-I; eIF4A-I; ATP-dependent RNA helicase eIF4A-1
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ImmunogenSynthetic Peptide of eIF4A1
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StorageMouse IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at +4°C short term. Store at -20°C long term. Avoid freeze / thaw cycle.
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PurificationAffinity-chromatography
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IsotypeMouse IgG1
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ClonalityMonoclonal
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DescriptionSwiss-Prot Acc.P60842.ATP-dependent RNA helicase which is a subunit of the eIF4F complex involved in cap recognition and is required for mRNA binding to ribosome. In the current model of translation initiation, eIF4A unwinds RNA secondary structures in the 5'-UTR of mRNAs which is necessary to allow efficient binding of the small ribosomal subunit, and subsequent scanning for the initiator codon.
Applications
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ReactivityHuman,Mouse,Rat
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ApplicationWB,IHC,ICC/IF
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Recommended dilutionWB: 1/500-1/1000 IHC: 1/50-1/100 ICC/IF: 1/50-1/200
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Western blot analysis of eIF4A1 in T47D,3T3,COS7,C6 and Hela lysates using eIF4A1 antibody. -
Immunohistochemistry analysis of paraffinembedded Human breast cancer tissue using eIF4A1 (6D7) antibody. -
Immunofluorescence analysis of eIF4A1 (6D7) in Hela using eIF4A1 (6D7) antibody(Left) ,and DAPI (blue).
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