Cleaved Caspase-8 (4C2) Mouse mAb_单克隆抗体-上海泊湾生物技术有限公司

Cleaved Caspase-8 (4C2) Mouse mAb

Source: Mouse mAb Catalog No. : AB3540

Cleaved Caspase-8 (4C2) Mouse mAb (AB3540)
UniProt:Q14790
Application:WB,IHC,ICC/IF
Reactivity:Human,Mouse,Rat
Source:Mouse mAbhttp://www.abways.cn/showproduct.asp?cid=AB3540

 Datasheet

数据表

UniProt

Q14790
Mol Weight

42,57kDa
Alternative Names

CASP8; MCH5; Caspase-8; CASP-8; Apoptotic cysteine protease; Mch-5; CAP4; FADD-homologous ICE/ced-3-like protease; FADD-like ICE; FLICE; ICE-like apoptotic protease 5; MORT1-associated ced-3 homolog; MACH; Cleaved Caspase-8 (Asp384)
Immunogen

Recombinant Protein of Caspase-8
Storage

Mouse IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at +4°C short term. Store at -20°C long term. Avoid freeze / thaw cycle.
Purification

Affinity-chromatography
Isotype

Mouse IgG1
Clonality

Monoclonal
Description

Swiss-Prot Acc.Q14790.Most upstream protease of the activation cascade of caspases responsible for the TNFRSF6/FAS mediated and TNFRSF1A induced cell death. Binding to the adapter molecule FADD recruits it to either receptor. The resulting aggregate called death-inducing signaling complex (DISC) performs CASP8 proteolytic activation. The active dimeric enzyme is then liberated from the DISC and free to activate downstream apoptotic proteases. Proteolytic fragments of the N-terminal propeptide (termed CAP3, CAP5 and CAP6) are likely retained in the DISC. Cleaves and activates CASP3, CASP4, CASP6, CASP7, CASP9 and CASP10. May participate in the GZMB apoptotic pathways. Cleaves ADPRT. Hydrolyzes the small-molecule substrate, Ac-Asp-Glu-Val-Asp-|-AMC. Likely target for the cowpox virus CRMA death inhibitory protein. Isoform 5, isoform 6, isoform 7 and isoform 8 lack the catalytic site and may interfere with the pro-apoptotic activity of the complex.

Applications

Reactivity

Human,Mouse,Rat
Application

WB,IHC,ICC/IF
Recommended dilution

WB: 1/500-1/1000 IHC: 1/50-1/100 ICC/IF: 1/50-1/200

Western blot analysis of Caspase8 in K562,3T3,MCF7 and Jurkat lysates using Caspase8 antibody.
Immunohistochemistry analysis of paraffinembedded Mouse Spleen Tissue using Caspase8 antibody.
Immunocytochemistry analysis of Caspase8 (4C2) (green) in Hela using Caspase8 (4C2) antibody, and DAPI(blue).
Western blot analysis of Caspase 8 (2G12) in THP1,Rat Brain,Hela lysates using Caspase8 (4C2) antibody.