Bcl-2 (3H5) Mouse mAb_单克隆抗体-上海泊湾生物技术有限公司

Bcl-2 (3H5) Mouse mAb

Source: Mouse mAb Catalog No. : AB3559

Bcl-2 (3H5) Mouse mAb (AB3559)
UniProt:P10415
Application:WB,IHC,ICC/IF
Reactivity:Human,Chicken
Source:Mouse mAbhttp://www.abways.cn/showproduct.asp?cid=AB3559

 Datasheet

数据表

UniProt

P10415
Mol Weight

26kDa
Alternative Names

BCL2; Apoptosis regulator Bcl-2
Immunogen

Synthetic Peptide of Bcl-2
Storage

Mouse IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at +4°C short term. Store at -20°C long term. Avoid freeze / thaw cycle.
Purification

Affinity-chromatography
Isotype

Mouse IgG1
Clonality

Monoclonal
Description

Swiss-Prot Acc.P10415.Suppresses apoptosis in a variety of cell systems including factor-dependent lymphohematopoietic and neural cells. Regulates cell death by controlling the mitochondrial membrane permeability. Appears to function in a feedback loop system with caspases. Inhibits caspase activity either by preventing the release of cytochrome c from the mitochondria and/or by binding to the apoptosis-activating factor (APAF-1). May attenuate inflammation by impairing NLRP1-inflammasome activation, hence CASP1 activation and IL1B release (PubMed:17418785).

Applications

Reactivity

Human,Chicken
Application

WB,IHC,ICC/IF
Recommended dilution

WB: 1/500-1/1000 IHC: 1/50-1/100 ICC/IF: 1/50-1/200

Western blot analysis of Bcl2 in human breast cancer cell line MCF7(A), MDAMB231(B) and Cal51(C) using Bcl2 antibody.
Immunohistochemistry analysis of paraffinembedded Human tonsil tissue using Bcl2 (3H5) antibody.
Immunohistochemistry analysis of paraffinembedded Human breast cancer Using Bcl2 antibody. Highpressure and temperature Citric acid pH6.0 was used for antigen retrieval.