Phospho-Vimentin (Ser83) Antibody
Source: Rabbit pAb Catalog No. : AY0267
Phospho-Vimentin (Ser83) Antibody (AY0267)
UniProt:P08670
Application:WB,IHC,ELISA
Reactivity:Human:S83,Mouse:S83,Rat:S83
Source:Rabbit pAbhttp://www.abways.cn/showproduct.asp?cid=AY0267
数据表
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UniProt
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Mol Weight
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Alternative NamesVIME
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ImmunogenThe antiserum was produced against synthesized phosphopeptide derived from human Vimentin around the phosphorylation site of serine 83 (Q-D-SP-V-D).
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StorageRabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at +4°C short term. Store at -20°C long term. Avoid freeze / thaw cycle.
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PurificationThe antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation
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IsotypeRabbit IgG
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ClonalityPolyclonal
Applications
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ReactivityHuman:S83,Mouse:S83,Rat:S83
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ApplicationWB,IHC,ELISA
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Recommended dilutionWB: 1:500~1:3000 IHC: 1:50~1:100 ELISA: 1:10000
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Western blot analysis of extracts from 293 cells, treated with paclitaxel (1uM, 24hours), using Vimentin (Phospho-Ser83) antibody. -
Immunohistochemistry analysis of paraffin-embedded human breast carcinoma tissue using Vimentin (Phospho-Ser83) antibody.
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