Phospho-p47 phox (Ser345) Antibody
Source: Rabbit pAb Catalog No. : AY0313
Phospho-p47 phox (Ser345) Antibody (AY0313)
UniProt:P14598
Application:WB,IHC
Reactivity:Human:S345
Source:Rabbit pAbhttp://www.abways.cn/showproduct.asp?cid=AY0313
数据表
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UniProt
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Mol Weight
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Alternative Names47 kDa autosomal chronic granulomatous disease protein; 47 kDa neutrophil oxidase factor; NCF-1; NCF-47K; NCF1; Neutrophil cytosol factor 1; Neutrophil cytosolic factor 1; Neutrophil NADPH oxidase factor 1; P47 phox; p47-phox
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ImmunogenThe antiserum was produced against synthesized phosphopeptide derived from human p47 phox around the phosphorylation site of serine 345 (P-Q-SP-P-G).
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StorageRabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at +4°C short term. Store at -20°C long term. Avoid freeze / thaw cycle.
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PurificationThe antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation
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IsotypeRabbit IgG
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ClonalityPolyclonal
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DescriptionNCF2, NCF1, and a membrane bound cytochrome b558 are required for activation of the latent NADPH oxidase (necessary for superoxide production).
Applications
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ReactivityHuman:S345
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ApplicationWB,IHC
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Recommended dilutionWB: 1:500~1:3000 IHC: 1:50~1:100 ELISA: 1:1000
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Western blot analysis of extracts from HepG2 cells, treated with TNF (20ng/ml, 5mins), using p47 phox (Phospho-Ser345) antibody. -
Immunohistochemistry analysis of paraffin-embedded human brain tissue using p47 phox (Phospho-Ser345) antibody.
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