Phospho-B-Raf (Thr753) Antibody
Source: Rabbit pAb Catalog No. : AY0319
Phospho-B-Raf (Thr753) Antibody (AY0319)
UniProt:P15056
Application:WB
Reactivity:Human:S753,Mouse:S790,Rat:S790
Source:Rabbit pAbhttp://www.abways.cn/showproduct.asp?cid=AY0319
数据表
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UniProt
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Mol Weight84 Kda
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Alternative NamesB-RAF; B-RAF proto-oncogene serine/threonine-protein kinase; B-Raf proto-oncogene serine/threonine-protein kinase; BRAF; BRAF1; c-RMIL; EC 2.7.1.37; EC 2.7.11.1; kinase B-Raf; p94; RAFB1; RMIL; RMIL serine/threonine-protein kinase; v-Raf murine sarcoma vi
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ImmunogenThe antiserum was produced against synthesized phosphopeptide derived from human B-Raf around the phosphorylation site of threonine 753 (P-K-TP-P-I).
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StorageRabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at +4°C short term. Store at -20°C long term. Avoid freeze / thaw cycle.
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PurificationAffinity-chromatography
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IsotypeRabbit IgG
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ClonalityPolyclonal
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DescriptionProtein kinase involved in the transduction of mitogenic signals from the cell membrane to the nucleus (Probable). Phosphorylates MAP2K1, and thereby activates the MAP kinase signal transduction pathway (PubMed:21441910, PubMed:29433126).
Applications
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ReactivityHuman:S753,Mouse:S790,Rat:S790
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ApplicationWB
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Recommended dilutionWB: 1:500~1:3000 ELISA: 1:5000
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Western blot analysis of extracts from K562 cells, treated with EGF (200ng/ml, 30mins), using B-Raf (Phospho-Thr753) antibody.
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