Phospho-NF-κB p100/p52 (Ser866) Antibody_多克隆抗体-上海泊湾生物技术有限公司

Phospho-NF-κB p100/p52 (Ser866) Antibody

Source: Rabbit pAb Catalog No. : AY0602

Phospho-NF-κB p100/p52 (Ser866) Antibody (AY0602)
UniProt:Q00653
Application:WB,IHC,IP,ELISA
Reactivity:Human:S866,Mouse:S865,Rat:S864
Source:Rabbit pAbhttp://www.abways.cn/showproduct.asp?cid=AY0602

 Datasheet

数据表

UniProt

Q00653
Mol Weight

100kDa
Alternative Names

NF-κB p100/p52;
Immunogen

The antiserum was produced against synthesized phosphopeptide derived from human NF-κB p100/p52 around the phosphorylation site of Serine 865.
Storage

Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at +4°C short term. Store at -20°C long term. Avoid freeze / thaw cycle.
Purification

Affinity-chromatography
Isotype

Rabbit IgG
Clonality

Polyclonal
Description

NFkB-p100 a transcription factor of the nuclear factor-kappaB ( NFkB) group. Precursor of the p52 subunit of the nuclear factor NF-kappa-B, which binds to the kappa-B consensus sequence 5'-GGRNNYYCC-3', located in the enhancer region of genes involved in immune response and acute phase reactions.

Applications

Reactivity

Human:S866,Mouse:S865,Rat:S864
Application

WB,IHC,IP,ELISA
Recommended dilution

WB: 1:500~1:3000 IHC: 1:50~1:100 ELISA: 1:20000 IP: Various Dilution

Western blot analysis of extracts from ovary cancer cells, using NF-κB p100/p52 (Phospho-Ser865) Antibody. Line1: Untreated; Line2: Treated with synthesized phosphopeptide.
Immunohistochemical analysis of paraffin- embedded breast carcinoma. Left: Using NF-κB p100/p52 (Phospho-Ser865) Antibody; Right: The same antibody preincubated with synthesized phosphopeptide.