RNF20 Antibody
Source: Rabbit mAb Catalog No. : CY5009
RNF20 Antibody (CY5009)
UniProt:Q5VTR2
Application:WB,ICC/IF,IP,FC
Reactivity:Human,Mouse
Source:Rabbit mAbhttp://www.abways.cn/showproduct.asp?cid=CY5009
数据表
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UniProt
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Mol Weight114kDa
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Alternative NamesBRE1-A; hBRE1; RING finger protein 20; E3 ubiquitin-protein ligase BRE1A; RNF20;
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ImmunogenA synthesized peptide derived from human RNF20
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StorageRabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at +4°C short term. Store at -20°C long term. Avoid freeze / thaw cycle.
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PurificationAffinity-chromatography
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IsotypeRabbit IgG
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ClonalityMonoclonal
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DescriptionRNF20 is an E3 ubiquitin ligase protein that mediates monoubiquitination of histone H2B and the methylation of histone H3. It forms a ubiquitin ligase complex in cooperation with the E2 enzyme UBE2E1/UBCH6. It thereby plays a central role in histone code and gene regulation. It is required for transcriptional activation of Hox genes.
Applications
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ReactivityHuman,Mouse
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ApplicationWB,ICC/IF,IP,FC
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Recommended dilutionWB 1:500~1:2000 ICC/IF 1:50~1:200 IP 1:50 FC 1:50
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All lanes use the Antibody at 1:1K dilution for 1 hour at room temperature. -
Immunofluorescent analysis using the Antibody at 1:50 dilution. -
Immunofluorescent analysis using the Antibody at 1:150 dilution. -
Western blot analysis of RNF20 expression in (1) 293 cell lysate; (2) HeLa cell lysate.
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