Smad3 Antibody
Source: Rabbit mAb Catalog No. : CY5013
Smad3 Antibody (CY5013)
UniProt:P84022
Application:WB,IHC,ICC/IF,FC
Reactivity:Human,Mouse,Rat
Source:Rabbit mAb
References（4）
http://www.abways.cn/showproduct.asp?cid=CY5013
数据表
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UniProt
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Mol Weight55kDa
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Alternative NamesJV15-2; MADH3; Mad3; Smad 3;
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ImmunogenA synthesized peptide derived from human Smad3
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StorageRabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at +4°C short term. Store at -20°C long term. Avoid freeze / thaw cycle.
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PurificationAffinity-chromatography
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IsotypeRabbit IgG
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ClonalityMonoclonal
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DescriptionSmad3 transcription factor phosphorylated and activated by TGF-beta-type receptors. A receptor-regulated Smad (R-smad). Binds directly to consensus DNA-binding elements in the promoters of target genes. In mouse required for establishemnt of the mucosal immune response and proper development of skeleton.
Applications
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ReactivityHuman,Mouse,Rat
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ApplicationWB,IHC,ICC/IF,FC
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Recommended dilutionWB 1:1000~1:5000 IHC 1:100~1:500 ICC/IF 1:100~1:500 FC 1:50
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All lanes use the Antibody at 1:5K dilution for 1 hour at room temperature. -
All lanes use the Antibody at 1:5K dilution for 1 hour at room temperature. -
All lanes use the Antibody at 1:5K dilution for 1 hour at room temperature. -
All lanes use the Antibody at 1:5K dilution for 1 hour at room temperature. -
Immunohistochemical analysis of paraffin-embedded Human lung large cell cancer, using the Antibody at 1:500 dilution. -
Immunohistochemical analysis of paraffin-embedded Human testis cancer, using the Antibody at 1:500 dilution. -
Immunohistochemical analysis of paraffin-embedded Human breast cancer, using the Antibody at 1:500 dilution. -
Immunofluorescent analysis using the Antibody at 1:150 dilution. -
Western blot analysis of Smad3 expression in (1) Jurkat cell lysate; (2) Rat liver lysate. -
Immunohistochemical analysis of paraffin-embedded mouse kidney, using Smad3 Antibody. -
Immunofluorescent analysis of Hela cells, using Smad3 Antibody.
引用文献（4条）
miR-744-5p suppresses tumor proliferation and metastasis by targeting transforming growth factor-beta 1 (TGF-β1) in hepatocellular carcinoma (HCC)-Journal of Gastrointestinal Oncology (J Gastrointest Oncol)
Sinomenine Relieves Airway Remodeling By Inhibiting Epithelial-Mesenchymal Transition Through Downregulating TGF-β1 and Smad3 Expression In Vitro and In Vivo.-Frontiers in Immunology (FRONT IMMUNOL)
SEPHS1 promotes SMAD2/3/4 expression and hepatocellular carcinoma cells invasion-Experimental Hematology & Oncology (EXP HEMATOL ONCOL)
AGGF1 therapy inhibits thoracic aortic aneurysms by enhancing integrin α7-mediated inhibition of TGF-β1 maturation and ERK1/2 signaling -nature communications
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