NF-κB p65 Antibody_单克隆抗体-上海泊湾生物技术有限公司

NF-κB p65 Antibody

Source: Rabbit mAb Catalog No. : CY5034

NF-κB p65 Antibody (CY5034)
UniProt:Q04206
Application:WB,IHC,ICC/IF,IP,FC
Reactivity:Human,Mouse
Source:Rabbit mAb
References（10）
http://www.abways.cn/showproduct.asp?cid=CY5034

 Datasheet

数据表

UniProt

Q04206
Mol Weight

65kDa
Alternative Names

NFKB3; Transcription factor p65; p65; Nuclear factor NF-kappa-B p65 subunit, RELA; TF65;
Immunogen

A synthesized peptide derived from human NF-κB p65
Storage

Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at +4°C short term. Store at -20°C long term. Avoid freeze / thaw cycle.
Purification

Affinity-chromatography
Isotype

Rabbit IgG
Clonality

Monoclonal
Description

NFKB1 (MIM 164011) or NFKB2 (MIM 164012) is bound to REL (MIM 164910), RELA, or RELB (MIM 604758) to form the NFKB complex. The p50 (NFKB1)/p65 (RELA) heterodimer is the most abundant form of NFKB. The NFKB complex is inhibited by I-kappa-B proteins (NFKBIA, MIM 164008 or NFKBIB, MIM 604495), which inactivate NFKB by trapping it in the cytoplasm.

Applications

Reactivity

Human,Mouse
Application

WB,IHC,ICC/IF,IP,FC
Recommended dilution

WB 1:5000~1:10000 IHC 1:50~1:100 ICC/IF 1:50~1:100

All lanes use the Antibody at 1:4W dilution for 1 hour at room temperature.
All lanes use the Antibody at 1:4W dilution for 1 hour at room temperature.
All lanes use the Antibody at 1:4W dilution for 1 hour at room temperature.
All lanes use the Antibody at 1:2W dilution for 1 hour at room temperature.
All lanes use the Antibody at 1:2W dilution for 1 hour at room temperature.
Immunofluorescent analysis using the Antibody at 1:50 dilution.
Western blot analysis of NF-κB p65 expression in HeLa cell lysate.
Immunohistochemical analysis of paraffin-embedded human transitional cell carcinoma of bladder, using NF-κB p65 Antibody.
Immunofluorescent analysis of HT-1080 cells, using NF-κB p65 Antibody .
Polysaccharides from Nostoc commune Vaucher activate macrophages via NF-κB and AKT/JNK1/2 pathways to suppress colorectal cancer growth in vivo. -Food & Function
JLX001 Modulated the Inflammatory Reaction and Oxidative Stress in pMCAO Rats via Inhibiting the TLR2/4-NF-κB Signaling Pathway. -Neurochemical Research
Celecoxib protects hyperoxia-induced lung injury via NF-κB and AQP1. -Frontiers in pediatrics
All lanes use the Antibody at 1: 5K dilution for 1 hour at room temperature.
Immunoprecipitate (IP) analysis using the Antibody at 1:50 dilution. (wb at 1:6K dilution)

引用文献（10条）

Moracin C, A Phenolic Compound Isolated from Artocarpus heterophyllus, Suppresses Lipopolysaccharide-Activated Inflammatory Responses in Murine Raw264.7 Macrophages.-International Journal of Molecular Sciences

Long-term di (2-ethylhexyl)-phthalate exposure promotes proliferation and survival of HepG2 cells via activation of NFκB.-Toxicology in Vitro

Celecoxib protects hyperoxia-induced lung injury via NF-κB and AQP1.-Frontiers in pediatrics

JLX001 Modulated the Inflammatory Reaction and Oxidative Stress in pMCAO Rats via Inhibiting the TLR2/4-NF-κB Signaling Pathway.-Neurochemical Research

Polysaccharides from Nostoc commune Vaucher activate macrophages via NF-κB and AKT/JNK1/2 pathways to suppress colorectal cancer growth in vivo.-Food & Function

更多+