ER alpha Antibody_单克隆抗体-上海泊湾生物技术有限公司

ER alpha Antibody

Source: Rabbit mAb Catalog No. : CY5041

ER alpha Antibody (CY5041)
UniProt:P03372
Application:WB,IHC,ICC/IF,FC,ChIP
Reactivity:Human,Mouse,Rat,Dog
Source:Rabbit mAbhttp://www.abways.cn/showproduct.asp?cid=CY5041

 Datasheet

数据表

UniProt

P03372
Mol Weight

60kDa
Alternative Names

ER; ESR; ESR1; Era; ESRA; NR3A1;
Immunogen

A synthesized peptide derived from human ER alpha
Storage

Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at +4°C short term. Store at -20°C long term. Avoid freeze / thaw cycle.
Purification

Affinity-chromatography
Isotype

Rabbit IgG
Clonality

Monoclonal
Description

ER (estrogen receptor 1) a member of the steroid receptor superfamily, contains highly conserved DNA binding (DBD) and ligand binding domains (LBD). Through its estrogen-independent and estrogen-dependent activation domains (AF-1 and AF-2, respectively), ER regulates transcription by recruiting coactivator proteins and interacting with general transcriptional machinery. Phosphorylation provides an important mechanism to regulate ER activity. ER is phosphorylated on multiple sites.

Applications

Reactivity

Human,Mouse,Rat,Dog
Application

WB,IHC,ICC/IF,FC,ChIP
Recommended dilution

WB 1:500~1:2000 IHC 1:50~1:100 ICC/IF 1:50~1:100 ChIP FC 1:30

All lanes use the Antibody at 1:1K dilution for 1 hour at room temperature.
All lanes use the Antibody at 1:1K dilution for 1 hour at room temperature.
All lanes use the Antibody at 1:1K dilution for 1 hour at room temperature.
All lanes use the Antibody at 1:500 dilution for 1 hour at room temperature.
All lanes use the Antibody at 1:500 dilution for 1 hour at room temperature.
Western blot analysis of ER alpha expression in (1) MCF7 cell lysate; (2)T47-D cell lysate.
Immunohistochemical analysis of paraffin-embedded human cervix carcinoma, using ER alpha Antibody.
Immunofluorescent analysis of MCF7 cells, using ER alpha Antibody .