ERK2 Antibody_单克隆抗体-上海泊湾生物技术有限公司

ERK2 Antibody

Source: Rabbit mAb Catalog No. : CY5045

ERK2 Antibody (CY5045)
UniProt:P28482
Application:WB,IHC,ICC/IF,IP,FC
Reactivity:Human,Mouse,Rat
Source:Rabbit mAbhttp://www.abways.cn/showproduct.asp?cid=CY5045

 Datasheet

数据表

UniProt

P28482
Mol Weight

41kDa
Alternative Names

ERK2; PRKM1; PRKM2; Mitogen-activated protein kinase 1; MAP kinase 1; MAPK 1; ERT1; ERT1; Extracellular signal-regulated kinase 2 (ERK-2); MAP kinase isoform p42 (p42-MAPK); Mitogen-activated protein kinase 2 (MAP kinase 2; MAPK 2); MAPK1
Immunogen

A synthesized peptide derived from human ERK2
Storage

Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at +4°C short term. Store at -20°C long term. Avoid freeze / thaw cycle.
Purification

Affinity-chromatography
Isotype

Rabbit IgG
Clonality

Monoclonal
Description

Act as an integration point for multiple biochemical signals, and are involved in a wide variety of cellular processes such as proliferation, differentiation, transcription regulation and development. The activation of ERK2 requires its phosphorylation by upstream kinases. ERK2 is located in the cytoplasm of resting cells and translocates into the nucleus upon extracellular stimuli by active transport of a dimer. ERK2 is essential for placental development and ERK2 in the trophoblast compartment may be indispensable for the vascularization of the labyrinth.

Applications

Reactivity

Human,Mouse,Rat
Application

WB,IHC,ICC/IF,IP,FC
Recommended dilution

WB 1:500~1:2000 IHC 1:50~1:200 ICC/IF 1:50~1:200 IP 1:40 FC 1:40

All lanes use the Antibody at 1:2K dilution for 1 hour at room temperature.
Immunohistochemical analysis of paraffin-embedded Rat lung, using the Antibody at 1:100 dilution.
Immunohistochemical analysis of paraffin-embedded Rat skin, using the Antibody at 1:100 dilution.
Immunohistochemical analysis of paraffin-embedded Human melanoma, using the Antibody at 1:100 dilution.
Immunohistochemical analysis of paraffin-embedded Human renal cancer, using the Antibody at 1:100 dilution.
Immunohistochemical analysis of paraffin-embedded Mouse ovary, using the Antibody at 1:100 dilution.
Immunofluorescent analysis using the Antibody at 1:50 dilution.
Western blot analysis of ERK2 in (1) A431 cell lysate; (2) HeLa cell lysate.
Immunohistochemical analysis of paraffin-embedded human gastric adenocarcinoma, using ERK2 Antibody.
Immunoprecipitate (IP) analysis using the Antibody at 1:50 dilution. (wb at 1:6K dilution)