HMGB1 Antibody_单克隆抗体-上海泊湾生物技术有限公司

HMGB1 Antibody

Source: Rabbit mAb Catalog No. : CY5060

HMGB1 Antibody (CY5060)
UniProt:P09429
Application:WB,IHC,ICC/IF,FC
Reactivity:Human,Mouse,Rat
Source:Rabbit mAbhttp://www.abways.cn/showproduct.asp?cid=CY5060

 Datasheet

数据表

UniProt

P09429
Mol Weight

25kDa
Alternative Names

HMGB1; HMG3; HMG1; SBP 1; Amphoterin;
Immunogen

A synthesized peptide derived from human HMGB1
Storage

Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at +4°C short term. Store at -20°C long term. Avoid freeze / thaw cycle.
Purification

Affinity-chromatography
Isotype

Rabbit IgG
Clonality

Monoclonal
Description

High mobility group (HMG) proteins 1 and 2 are ubiquitous non-histone components of chromatin. Evidence suggests that the binding of HMG proteins to DNA induces alterations in the DNA architecture including DNA bending and unwinding of the helix. HMG proteins synergize with Oct-2, members of the NFκB family, ATF-2 and c-Jun to activate transcription.

Applications

Reactivity

Human,Mouse,Rat
Application

WB,IHC,ICC/IF,FC
Recommended dilution

WB 1:1000~1:2000 IHC 1:50~1:100 ICC/IF 1:50~1:100 FC 1:30

All lanes use the Antibody at 1:5W dilution for 1 hour at room temperature.
All lanes use the Antibody at 1:5W dilution for 1 hour at room temperature.
All lanes use the Antibody at 1:5W dilution for 1 hour at room temperature.
All lanes use the Antibody at 1:5W dilution for 1 hour at room temperature.
All lanes use the Antibody at 1:5W dilution for 1 hour at room temperature.
All lanes use the Antibody at 1:5W dilution for 1 hour at room temperature.
All lanes use the Antibody at 1:5W dilution for 1 hour at room temperature.
All lanes use the Antibody at 1:10W dilution for 1 hour at room temperature.
Immunohistochemical analysis of paraffin-embedded Rat liver, using the Antibody at 1:300 dilution.
Immunohistochemical analysis of paraffin-embedded Rat kidney, using the Antibody at 1:300 dilution.
Immunohistochemical analysis of paraffin-embedded Human non-Hodgkin's lymphoma, using the Antibody at 1:100 dilution.
Immunohistochemical analysis of paraffin-embedded Human testis cancer, using the Antibody at 1:100 dilution.
Immunohistochemical analysis of paraffin-embedded Mouse skeletal muscle - gastrocnemius , using the Antibody at 1:300 dilution.
Immunofluorescent analysis using the Antibody at 1:150 dilution.
Western blot analysis of HMGB1 expression in (1)HeLa cell lysate; (2)Rat brain lysate.
Immunohistochemical analysis of paraffin-embedded human kidney, using HMGB1 Antibody.
Immunofluorescent analysis of SW480 cells, using HMGB1 Antibody.