PMS2 Antibody
Source: Rabbit mAb Catalog No. : CY5114
PMS2 Antibody (CY5114)
UniProt:P54278
Application:WB,IHC,ICC/IF,IP,FC
Reactivity:Human
Source:Rabbit mAbhttp://www.abways.cn/showproduct.asp?cid=CY5114
数据表
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UniProt
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Mol Weight110kDa
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Alternative NamesDNA mismatch repair gene; DNA mismatch repair protein PMS2; HNPCC4; PMS1 protein homolog 2;
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ImmunogenA synthesized peptide derived from human PMS2
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StorageRabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at +4°C short term. Store at -20°C long term. Avoid freeze / thaw cycle.
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PurificationAffinity-chromatography
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IsotypeRabbit IgG
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ClonalityMonoclonal
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DescriptionAssembly of the MutL-MutS-heteroduplex ternary complex in presence of RFC and PCNA is sufficient to activate endonuclease activity of PMS2. It introduces single-strand breaks near the mismatch and thus generates new entry points for the exonuclease EXO1 to degrade the strand containing the mismatch. DNA methylation would prevent cleavage and therefore assure that only the newly mutated DNA strand is going to be corrected.
Applications
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ReactivityHuman
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ApplicationWB,IHC,ICC/IF,IP,FC
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Recommended dilutionWB 1:500~1:2000 IHC 1:50~1:200 ICC/IF 1:50~1:200 IP 1:50 FC 1:50
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All lanes use the Antibody at 1:5K dilution for 1 hour at room temperature. -
Immunofluorescent analysis using the Antibody at 1:50 dilution. -
Western blot analysis of PMS2 in (1) Jurkat cell lysate; (2)HeLa cell lysate. -
Immunohistochemical analysis of paraffin-embedded human tonsil, using PMS2 Antibody. -
Immunoprecipitate (IP) analysis using the Antibody at 1:50 dilution. (wb at 1:3K dilution)
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