Vimentin Antibody
Source: Rabbit mAb Catalog No. : CY5134
Vimentin Antibody (CY5134)
UniProt:P08670
Application:WB,IHC,ICC/IF,FC
Reactivity:Human,Mouse,Rat
Source:Rabbit mAb
References（13）
http://www.abways.cn/showproduct.asp?cid=CY5134
数据表
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UniProt
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Mol Weight54kDa
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Alternative NamesFLJ36605; VIM; VIME; Vimentin
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ImmunogenA synthesized peptide derived from human Vimentin
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StorageRabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at +4°C short term. Store at -20°C long term. Avoid freeze / thaw cycle.
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PurificationAffinity-chromatography
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IsotypeRabbit IgG
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ClonalityMonoclonal
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DescriptionVimentins are class-III intermediate filaments found in various non-epithelial cells, especially mesenchymal cells. Vimentin is attached to the nucleus, endoplasmic reticulum, and mitochondria, either laterally or terminally. Involved with LARP6 in the stabilization of type I collagen mRNAs for CO1A1 and CO1A2.
Applications
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ReactivityHuman,Mouse,Rat
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ApplicationWB,IHC,ICC/IF,FC
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Recommended dilutionWB 1:1000~1:5000 IHC 1:200~1:1000 ICC/IF 1:50~1:200 FC 1:50
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All lanes use the Antibody at 1:5K dilution for 1 hour at room temperature. -
All lanes use the Antibody at 1:6K dilution for 1 hour at room temperature. -
All lanes use the Antibody at 1:6K dilution for 1 hour at room temperature. -
Immunohistochemical analysis of paraffin-embedded Human lung large cell cancer, using the Antibody at 1:1000 dilution. -
Immunohistochemical analysis of paraffin-embedded Human lung adenocarcinoma, using the Antibody at 1:1000 dilution. -
Western blot analysis of Vimentin expression in HEK293 cell lysate. -
Immunohistochemical analysis of paraffin-embedded human colon, using Vimentin Antibody. -
Immunofluorescent analysis of Hela cells, using Vimentin Antibody. -
Prostaglandin E1 Inhibited Diabetes-Induced Phenotypic Switching of Vascular Smooth Muscle Cells Through Activating Autophagy. -Cellular Physiology and Biochemistry -
Overexpression of the 14-3-3γ protein in uterine leiomyoma cells results in growth retardation and increased apoptosis. -Cellular Signalling -
All lanes use the Antibody at 1:6K dilution for 1 hour at room temperature.
引用文献（13条）
Overexpression of the 14-3-3γ protein in uterine leiomyoma cells results in growth retardation and increased apoptosis.-Cellular Signalling
Prostaglandin E1 Inhibited Diabetes-Induced Phenotypic Switching of Vascular Smooth Muscle Cells Through Activating Autophagy.-Cellular Physiology and Biochemistry
Contribution of acid sphingomyelinase to angiotensin II-induced vascular adventitial remodeling via membrane rafts/Nox2 signal pathway.-Life Sciences
Inhibition of vascular adventitial remodeling by netrin-1 in diabetic rats-J Biol Chem (JOURNAL OF BIOLOGICAL CHEMISTRY)
Molecular hydrogen regulates PTEN‐AKT‐mTOR signaling via ROS to alleviate peritoneal dialysis‐related peritoneal fibrosis-FASEB JOURNAL (FASEB J)
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