Phospho-Smad3 (S423 + S425) Antibody_单克隆抗体-上海泊湾生物技术有限公司

Phospho-Smad3 (S423 + S425) Antibody

Source: Rabbit mAb Catalog No. : CY5140

Phospho-Smad3 (S423 + S425) Antibody (CY5140)
UniProt:P84022
Application:WB,IHC,ICC/IF
Reactivity:Human,Mouse
Source:Rabbit mAb
References（4）
http://www.abways.cn/showproduct.asp?cid=CY5140

 Datasheet

数据表

UniProt

P84022
Mol Weight

55kDa
Alternative Names

JV15-2, MAD-3, MADH3, Mad3, Mothers against DPP homolog 3, Mothers against decapentaplegic homolog 3, SMAD 3, Smad 3
Immunogen

A synthesized peptide derived from human Phospho-Smad3 (S423 + S425)
Storage

Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at +4°C short term. Store at -20°C long term. Avoid freeze / thaw cycle.
Purification

Affinity-chromatography
Isotype

Rabbit IgG
Clonality

Monoclonal
Description

Receptor-regulated SMAD (R-SMAD) that is an intracellular signal transducer and transcriptional modulator activated by TGF-beta (transforming growth factor) and activin type 1 receptor kinases. Binds the TRE element in the promoter region of many genes that are regulated by TGF-beta and, on formation of the SMAD3/SMAD4 complex, activates transcription.

Applications

Reactivity

Human,Mouse
Application

WB,IHC,ICC/IF
Recommended dilution

WB 1:500~1:2000 IHC 1:50~1:200 ICC/IF 1:50~1:200

All lanes use the Antibody at 1:1K dilution for 1 hour at room temperature.
All lanes use the Antibody at 1:1K dilution for 1 hour at room temperature.
All lanes use the Antibody at 1:1K dilution for 1 hour at room temperature.
All lanes use the Antibody at 1:1K dilution for 1 hour at room temperature.
Western blot analysis of Phospho-Smad3 (S423/S425) expression in A549 cell lysate treated with TGF-ß1.
Immunohistochemical analysis of paraffin-embedded human gastric adenocarcinoma, using Phospho-Smad3 (S423 + S425) Antibody.
Immunofluorescent analysis of A549 cells treated with TGFβ , using Phospho-Smad3 (S423 + S425) Antibody.
Immunofluorescent analysis using the Antibody at 1:50 dilution.

引用文献（4条）

MiR-20a-3p regulates TGF-β1/Survivin pathway to affect keratinocytes proliferation and apoptosis by targeting SFMBT1 in vitro.-Cellular Signalling

miR-744-5p suppresses tumor proliferation and metastasis by targeting transforming growth factor-beta 1 (TGF-β1) in hepatocellular carcinoma (HCC)-Journal of Gastrointestinal Oncology (J Gastrointest Oncol)

Ma Xing Shi Gan Decoction Protects against PM2.5-Induced Lung Injury through Suppression of Epithelial-to-Mesenchymal Transition (EMT) and Epithelial Barrier Disruption-Evidence-Based Complementary and Alternative Medicine (EVID-BASED COMPL ALT)

AGGF1 therapy inhibits thoracic aortic aneurysms by enhancing integrin α7-mediated inhibition of TGF-β1 maturation and ERK1/2 signaling -nature communications