Vinculin Antibody
Source: Rabbit mAb Catalog No. : CY5164
Vinculin Antibody (CY5164)
UniProt:P18206
Application:WB,ICC/IF,IP,
Reactivity:Human,Mouse,Rat
Source:Rabbit mAb
References（1）
http://www.abways.cn/showproduct.asp?cid=CY5164
数据表
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UniProt
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Mol Weight125kDa
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Alternative NamesVinculin;VCL;Metavinculin; MV;
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ImmunogenA synthesized peptide derived from human Vinculin
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StorageRabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at +4°C short term. Store at -20°C long term. Avoid freeze / thaw cycle.
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PurificationAffinity-chromatography
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IsotypeRabbit IgG
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ClonalityMonoclonal
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DescriptionVinculin is a cytoskeletal protein that plays an important role in the regulation of focal adhesions and embryonic development. Three structural vinculin domains include an amino-terminal head, a short, flexible proline-rich region and a carboxy-terminal tail. In the inactive state, the head and tail domains of vinculin interact to form a closed confirmation. The open and active form of vinculin translocates to focal adhesions where it is thought to be involved in anchoring F-actin to the membrane and regulation of cell migration.
Applications
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ReactivityHuman,Mouse,Rat
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ApplicationWB,ICC/IF,IP,
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Recommended dilutionWB 1:10000~1:20000 ICC/IF 1:50~1:200 IP 1:50
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All lanes use the Antibody at 1:5W dilution for 1 hour at room temperature. -
All lanes use the Antibody at 1:5W dilution for 1 hour at room temperature. -
All lanes use the Antibody at 1:5W dilution for 1 hour at room temperature. -
Western blot analysis of Vinculin expression in (1) HepG2 cell lysate; (2) HeLa cell lysate. -
Immunofluorescent analysis of A673 cells, using Vinculin Antibody .
引用文献（1条）
Genipin improves reproductive health problems caused by circadian disruption in male mice-Reproductive Biology and Endocrinology (REPROD BIOL ENDOCRIN)
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